Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Epidermal growth factor receptor ( EGFR ), cyclin dependent kinase 4 ( CDK4 ), mouse double minute 4 ( MDM4 ), and platelet-derived growth factor receptor alpha ( PDGFRA ) gene expression levels in glioblastoma multiforme (GBM) tissues from our series (assessed by RT-QPCR) and from the Gene Expression Omnibus (GEO) database assessed with the HGU133Plus2 Affymetrix microarray.

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: Expressing, Microarray

Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Epidermal growth factor receptor ( EGFR ), cyclin dependent kinase 4 ( CDK4 ), mouse double minute 4 ( MDM4 ), and platelet-derived growth factor receptor alpha ( PDGFRA ) gene expression levels in GBM ( n = 83) vs. normal brain tissues ( n = 42). (Non-parametric comparisons performed using the Mann–Whitney U test). (SPSS 25.0, IBM SPSS, Armonk, NY, USA).

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: Derivative Assay, Expressing, MANN-WHITNEY

Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Distribution of GBM according to the gene expression profiles (GEP) observed for the EGFR , CDK4 , MDM4 , and PDGFRA genes.

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: Expressing

Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Distribution of GBM cases according to the pattern ( A ) and number ( B ) of overexpressed ( EGFR , CDK4 , MDM4 , and PDGFRA ) genes, and the association between EGFR gene expression levels and the EGFR gene deletion/amplification profiles ( C ).

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: Expressing, Amplification

Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Association between the copy number (CN) status and gene expression profile of the EGFR , CDK4 , MDM4 , and PDGFRA genes in GBM.

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: Expressing

Journal: Cancers

Article Title: Heterogeneous EGFR , CDK4 , MDM4 , and PDGFRA Gene Expression Profiles in Primary GBM: No Association with Patient Survival

doi: 10.3390/cancers12010231

Figure Lengend Snippet: Prognostic impact of EGFR ( A ), CDK4 ( B ), MDM4 ( C ), and PDGFRA ( D ) GEP on overall survival of GBM patients.

Article Snippet: Subsequently, cDNA was synthesized from total RNA (2 µg in 20 µL) treated with 1 µg DNase I (Sigma-Aldrich-Merck, Kenilworth, NJ, UK) using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, USA). cDNA was used for EGFR , PDGFRA , CDK4 , and MDM4 gene expression analysis based on an RQ-PCR assay and the BioMark HD System (Fluidigm, South San Francisco, CA, USA), using predesigned FAM-MGB labeled TaqMan ® probes (Thermo-Fisher Scientific, Waltham, MA, USA) for the EGFR (hs00193306_m1), PDGFRA (hs00998018_m1), CDK4 (hs00364847_m1), and MDM4 (Hs00967245_m1) genes ( ).

Techniques: